I want to study the effect of inhibition of proteosomal degradation machinery on the expression of a particular Transcription factor which we assume is degraded by ubiquitin mediated degradation on MCF-7 and A549 cells. What concentration of MG132 should I use and how long should I give the treatment. Your feedback will be valuable. Thanks!
We have never used MG132 in cell lines, but the concentration range we used in retinal tissue explants long ago may help design your pilot experiments.
See: Neves DD, Rehen SK, Linden R. Differentiation-dependent sensitivity to cell death induced in the developing retina by inhibitors of the ubiquitin-proteasome proteolytic pathway. Eur J Neurosci. 2001 May;13(10):1938-44
Rafael Linden Thank you for taking out the time to answer this question. I will look into it.
Hi Vishal,
concentrations around 1-25 micromolar and treatments from 2-12 hours should be OK. If you want a starting point I would use 10 micromolar for 4 hours
Hipe ir helps
Francisco
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