Vishal Sah

4 Questions 4 Answers 0 Followers

Questions related from Vishal Sah

Marker for mice primary Hepatocytes?

I am isolating primary hepatocytes from mice. I am using the two-step collagenase perfusion method and after seeding the cells in the 12-well plate, I am seeing two types of cell morphologies, one...

05 January 2022 6,278 1 View

Anyone having experience in generating Knockout huh-7 cells?

I want to try knocking out a gene in huh-7 cell lines. Does anyone have any prior experience in it? What difficulties should I expect and what protocols should I follow. Any recommendations for...

02 June 2021 2,982 0 View

What to use as a positive control for MG132 inhibition experiments?

I am trying to see the rescue effect of a particular protein upon inhibition of proteasome by MG132. I want a positive control to check if the inhibition happened properly. Can anyone suggest me a...

24 February 2020 9,314 2 View

What is the optimal concentration and incubation time for MG132 inhibition studies?

I want to study the effect of inhibition of proteosomal degradation machinery on the expression of a particular Transcription factor which we assume is degraded by ubiquitin mediated degradation...

06 December 2019 8,479 3 View