23 March 2024 1 4K Report

I have used ethanolic hcl reagent (95% ethanol and 1.5N Hcl in 85:15 ratio) for sample extraction. I got OD value at the range of 0.031 to 0.068.

formula used :

Total Anthocyanin mg/100g) = ( OD x dilution x total volume made up x 100 )/ wt of sample x e

e : 98.2, absorbance of a solution containing 1 mg/ml anthocyanin.

But, resulted values are in low range. Please suggest me regarding this

Thank you

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