I have used ethanolic hcl reagent (95% ethanol and 1.5N Hcl in 85:15 ratio) for sample extraction. I got OD value at the range of 0.031 to 0.068.
formula used :
Total Anthocyanin mg/100g) = ( OD x dilution x total volume made up x 100 )/ wt of sample x e
e : 98.2, absorbance of a solution containing 1 mg/ml anthocyanin.
But, resulted values are in low range. Please suggest me regarding this
Thank you