I've just started working with HMC3. Bought from ATTC, using MEM with 10% FBS 1%P/S. I split them last week when they had reached 80%. They do not seem to be growing but for the most part look healthy, although I do see some floating cells. I was told not to change media as often as the ATTC guidelines suggest, will this slow down division?
Any help would be great!
Hi Amy
I've been working with HMC-3 cells, the cells grows well in MEM, 10% FBS media. Post reviving it takes 2-3 passages for the cells to attain proper growth. Just we should ensure that the cells always should be passaged in high confluency or it will affect its morphology and media should be conditioned in the incubator before using it for the cells. The cells generally attain 80-90% confluency in 2 days.
Hope it works
Thanks
Hi,
I worked with HMC-3 cells before. Their growth rate was really slow for the first couple of weeks when I first thawed them. Then, it took 3-4 days to reach %80-90 confluency under the same conditions (same media and supplements). My suggestion is to maintain two different passages at the same time. For example, you may passage one group Monday, and the other group Wednesday. So, you can use the confluent cells for your experiment, while the other passage grows.
I don't know about cell division but if the cell media doesn't turn yellow, then you may not need to change media. Cells release some soluble factors and metabolites for cellular communication and their growth. So, when you remove cell media, you also remove these components. That might be the reason for the recommendation of not refreshing the media very often.
If you have just started to work with HMC3 cells, this article might be helpful. Article The human microglial HMC3 cell line: Where do we stand? A sy...
I hope this helps.
Best,
Thanks.