How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

In SPSS, how to select cases if variable has x but not y?

Hi all, I have an SPSS longitudinal database in which one of the variables is monthnumber. The month ranges from 0 (baseline) through all of the months they were involved and then 51 for last...

30 November 2020 490 4 View

What is the best way to analyse rainfall data against a second variable?

I am looking at how rainfall can influence volcanic activity. I have rainfall data from three different datasets. One is a gridded dataset at 0.25 x 0.25 degree resolution that offers monthly...

04 November 2020 5,970 3 View

Two peaks in an antibody purification through AKTA purifier?

When purifying monoclonal antibodies from hybridome cultures (protein G affinity chromatography) we got two different peaks, which we collected separately. When analysed by SDS-PAGE they both...

06 September 2020 3,662 5 View

How do I merge variables in SPSS?

I merged two datasets with different cases; one dataset has two cohorts/comparison groups and the other dataset has one cohort, the experimental group. Some of the variables are named the same and...

19 August 2020 5,150 3 View

Can I still identify a specific cell type by flow cytometry if the cells are dead or do I need to pre-sort to study cell death of a given population?

Hi everyone! I want to study the response of plasma cells to different drugs after in vitro exposure. Can I culture whole bone marrow (after RBC lysis), treat with my drug, wait an appropriate...

03 August 2020 8,194 6 View

Is is viable to conduct a content analysis on social media posts?

I'm intending on conducting a content analysis on the stigmatising effects of a recent anti-obesity campaign. I would like to do this analysing the comments in response to Instagram posts by a...

06 April 2020 5,341 10 View

Cable stayed bridge cable to deck connection and column?

What is a suitable connection and any good websites to explain the connection of the cables to the deck surface and the cables to the column on a single column cable stayed bridge.

06 December 2019 3,244 2 View

How to detect the mechanical property of powders by nanoindentation?

Hi everyone. Does anyone know how to hold the powders (10um) when doing nanoindentation? I have mixed the powders with epoxy, and let it dry, then polished the mixture, but I never got converged...

14 October 2019 3,100 5 View

Architectural Engineering research topic that links with interior design?

Looking for a topic for my dissertation that could link architectural engineering and interior design together. I'm thinking of studying interior design after architectural engineering and would...

18 August 2019 6,993 10 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View