How to plot upper bound and lower bound limit curves of trendline in MS Excel?

To obtain upper and lower bound curves for trendline of scattered plot.

10 May 2020 2,117 1 View

How to use Sephadex Column to isolate aminoglycoside compounds?

There are several types of Sephadex and they can be used in several manners for different compounds. I want to use Sephadex column to isolate aminoglycoside. If anyone having idea, how to use...

21 April 2020 2,478 4 View

Why am I getting the higher COD after applying AOP (O3+ H2O2)?

I am treating the industrial effluent by AOP using H2O2+ O3. I am getting higher COD values than raw wastewater itself. I quenched the H2O2 by adding MnO2. Samples were filtered 0.2-micrometer...

24 November 2019 3,117 8 View

The solubility of Acetylated paramomycin salt is in H2O only. How to isolate Acetylated Paramomycin salt synthesized from Paramomycin Sulfate salt?

Column chromatography is the technique to isolate the organic compound which is soluble in organic solvent. And I want to isolate a compound which is soluble only in water. Any technique or...

20 October 2019 9,207 1 View

Does keeping tissue sample (patient biopsies) in Trizol for a long time (>6 month) degrades DNA ? or Leads to sheared DNA?

I am trying to isolate RNA and DNA from the tissue biopsy stored in Trizol at -80 degree for more than 6 months . I isolated RNA using the standard Trizol method and obtained good quality and...

22 November 2018 2,446 2 View

Why are my gel pieces not getting destained for in-gel digestion?

My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.

07 May 2018 5,419 1 View

Is it possible to find the sensitivity of RMSD and RMSF in MD simulation through gromacs ?

Is it possible to find the regions of the protein structures most susceptible to temperature impact in terms of RMSD and RMSF , during MD simulation ?

06 May 2018 8,855 3 View

How to properly store the sds gel pieces for in gel digestion?

I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?

15 January 2018 1,120 1 View

Is proteomic data analysis in R (using RforProteomics) better than any other proteomic software?

My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?

04 January 2018 2,591 6 View

I want reference of first paper of NiO/ZnO nanocomposites?

i want to know from were work on ZnO/NiO nanocomposite had started.

26 September 2017 3,538 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View