Negative correlation interpretation?

How would you describe the relationship between two scores with an r obtained of -.312, with a p value = .000?

25 November 2018 6,909 7 View

Can we use Pearson's r for determining the relationship of two variables with instruments using the likert scale?

Our thesis research has the following questions: 1. What is their level of (variable a)? 2. What is their level of (variable b)? 3. Which of the five components of (variable b) relate to...

04 November 2018 2,825 4 View

Does anyone have a copy of the questionnaire and all the psychometric properties of workplace deviance?

This particular scale is needed for my thesis. Thank you very much.

09 September 2018 6,117 3 View

If I have a concetration 100ug/ul, How do I do for obtain 50ng to PCR?

24 June 2018 5,963 7 View

Has anyone done a two plasmid simultaneous transformation in a chemically competent E. coli?

05 August 2017 2,679 6 View

Spectinomycin (LB medium) concentration

30 May 2017 1,499 4 View

What is maximum amount of plasmids, i could insert in a host cell? Why? Also where can i find some useful information about plasmids compatibility?

17 March 2017 7,275 4 View

How to get curve fit error range, from data with confidence interval?

03 April 2016 9,882 0 View

What is the most suitable neuroblastoma cell line for studies related to the expression of metabotropic glutamate receptors (mGluRs)?

I would like start a project with neuronal cell culture. I am looking for a neuroblastoma cell line with characteristics of dopaminergic and/or glutamatergic neurons and expressing mGluRs. What is...

19 January 2016 8,130 1 View

Does anyone have experience in culture HL-60 cell line in a serum free medium or lipid-reduced FBS ?

The main goal is get rid of lipids and fatty acids contents in the FBS.

06 April 2015 1,255 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View