My organism is an obligate anearobe but aerotolerant, Hence a certain degree of anaerobic conditions has to be maintained.
I am well aware of the medium requirements of my organism and the basic necessity of a reducing medium.
But I want an insight into a practical suggestion with flask preparation, maintenance, subculturing, sampling, etc.
Thank you
Dear- Nikunj Daga, Personally what I can say from my research experience is to grow an anaerobic bacteria you need to stop or block airway through which air comes. This you can do by putting a piece of cotton in the mouth of the flask followed by covering of Shake flask with a transparent cellophane sheet. And as you said your bacteria is obligate anaerobe so you also need some supply of air, so in that case only cotton will work perfect practically.
hello Nikunj Daga I think you can grow in jars with screw caps containing fresh medium thyoglycolate and this will grow in the bottom of it
Many obligate anaerobes can tolerate some exposure to oxygen but would be stressed. I would think that depending on your research goals etc, you would be best to grow them in serum bottles under a nitrogen atmosphere with septum caps. If you want to agitate them you can still do it of course. Sampling is done with sterile needle and syringe.
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