Where can I get LN-308 cell line?

Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...

20 October 2020 3,004 3 View

How can I quantify bacteria viable cells in a non-sterilized organic compost?

I'm working with the enrichment of an organic compost with PGPR, but this compost is not sterilized. How could I quantify viable cells of Azospirillum brasilense, Bacillus subtilis, and...

01 April 2020 9,486 4 View

How to model near-stall compressor operation with RANS CFD?

Hello everyone. I am looking into near-stall behaviour of the multistage axial compressor using CFD with RANS method. What is the best practice to establish if the compressor is close to stall,...

09 March 2020 8,847 8 View

What iron species are precipitating after autoclaving ferric chloride water solution?

I made 10mM FeCl3 solution in MQ water. After autoclaving it (standard 15min procedure) solution turns orange-brown. Filtering using 0.22um filter does not remove the color (but some of it is...

24 September 2019 2,407 7 View

What is a typical RFU value for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer?

Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...

10 April 2018 5,090 3 View

RNAprotect reagent formulation?

Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...

04 March 2018 5,089 14 View

How much does ammonium sulfate precipitation affect nucleic acid integrity?

Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...

04 March 2018 1,589 3 View

SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data and Light Pollution Assessment

We are working with SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data, which has units of W cm-2 sr-1. The case study area is a bird sanctuary and an inhabited territory that is also used for...

14 May 2017 7,715 2 View

Is there a relation between Hadamard product and standard matrix product?

05 July 2015 5,366 7 View

Can we get the sufficient number of intensity measurements for a complex vector reconstruction in quantum tomography?

In a paper arXiv:1312.0158 the authors proved that any complex vector (thus also the quantum state vector) can be uniquely determined (up to a global phase factor) by 4N-4 generic intensity...

22 June 2015 7,125 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View