hello everyone, i am doing some research about DC vaccine for liver cancer. my plan is pulse DC with specific antigens and then immunized mouse 2 times at one week interval, after the second immunization, isolate murine's spleenocytes and then co-culture with mitomycin C treated target cells for 6-7days. but i don't know what is the approximate working concentration range of mitomycin C and how long i should use it to treat my target cells. i hope people who know it could give me some advice. i really appreicate that !