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Questions related from Han Zhang
hi every one, i want to isolate antigen-specific CTL. my plan is as follows: 1. immunize mouse with peptide twice, at 2 weeks interval; 2. after 2weeks of second immunization, kill mouse and...
16 March 2020 4,373 3 View
hello everyone, i am doing some research about DC vaccine for liver cancer. my plan is pulse DC with specific antigens and then immunized mouse 2 times at one week interval, after the second...
06 December 2019 5,393 2 View
recently , i have found an interesting phenomenon when i did some experiment about murine Treg. my protocol as follows: first of all i use cell sorting kit isolate cd4+cd25+cells(i consider them...
13 August 2019 4,685 3 View
Is there a website that can be used to query the HLA typing of tumor cell lines? can anybody give me any advice?
01 July 2019 2,923 5 View
I want to use anti-CD3 and anti-cd28 stimulate Treg, i noticed that some researcher use soluble antiCD3, while others use plate-bound anti-CD3, i don't know what is the difference bewteen soluble...
22 May 2019 3,637 1 View
I am planing to test Treg cells proliferation after activated by TLR2 agonists. I have read some papers and found that some researcher use [3H]thymidine incorporation as a readout, while in the...
09 May 2019 9,103 3 View
I intend to co-culture BMDC with irradiated B16 cells as a vaccine fto prevent B16 tumorigenesis. I need to immunize four times,but it is very inconvenient to treat tumor cells with radiation each...
28 March 2019 6,404 2 View
