Rice is a diploid species with 12 chromosomes. I plan to use a BC₂F₃ population of 100–130 individuals for mapping drought-associated QTLs. What is the minimum number of polymorphic markers and the ideal population size recommended for this project to ensure reliable QTL detection?
Minimum Number of Polymorphic Markers
Ideal Population Size
Considerations for QTL Mapping
- Genetic Variability: Ensure that the markers used are highly polymorphic within the mapping population. This variability is crucial for distinguishing between different genetic backgrounds and accurately identifying QTLs.
- Mapping Strategy: Utilize a high-density marker set and advanced statistical methods for QTL analysis to increase the reliability and resolution of your QTL mapping.
Summary
- Markers: Aim for a minimum of 100–150 polymorphic markers, ideally distributed across the genome with a spacing of 5–20 cM.
- Population Size: A population of 100–130 individuals is suitable, but a larger population can provide more robust and precise results.
Ideally, there should be one marker after every 5cM for robust results in Qtl mapping. The higher density of the polymorphic marker gives better resolution and precision.
It's recommended to use 100–200 polymorphic markers to ensure adequate coverage of the genome. Opting for a population size closer to 130 individuals will enhance the power of detecting QTLs, making this approach a solid foundation for identifying drought-associated traits.
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