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Questions related from Chandima Ariyarathna
Please suggest a tool to estimate linkage disequilibrium in haploid organisms like fungi
12 March 2024 9,336 3 View
Please help me understanding the difference in Binary Diploid, Binary Haploid, and Haploid data types in GenAlex 6.5. I want use a binary dataset (RAPD) from fungal species which has a haploid...
15 February 2024 9,248 3 View
Magnaporthe grisea and M. oryza are heterothallic fungi. Mating type analysis using standard primer sets (Bao-Hua et, 2004) however, identify duel maters meaning the presence of both MAT1-1 and...
20 July 2022 2,034 0 View
Hi I need to label 2 branches in a phylogenetic tree. Can somebody tell me if I have labelled the branches in these newick trees correctly. I need to use these trees in PAML to see positive...
05 January 2016 4,543 2 View
Hi, I am interested in an ortholog of rice OsHKT1;4 in wheat and found only a partial sequence (3' end) of the wheat gene on 2AL using IWGS survey sequence database. This gene is expressed through...
16 June 2015 3,543 7 View
Hi, I made a sequence comparison of 5' UTR region of a membrane transporter protein gene (HKT) between rice and wheat . Around 500bp upstream there is 200bp tandem duplication. Is this a simple...
31 December 2014 4,914 3 View
I queried a few (QTL) markers in GrainGene DB
26 June 2013 911 0 View
The gene I am working on has 6 copies. I have retrieved genomic DNA sequences of all of them from public domain. I also managed to get FL-cDNA of 5 of them. The missing one (I call it A-2) has...
20 May 2013 4,017 13 View
I have designed genome specific primers for a Na+ transporter gene in wheat. The primers work well on genomic DNA but on cDNA as a template they produce non-specific bands. I have synthesized...
30 March 2013 6,447 4 View