What is the minimum concentration of ethanol needed to trigger the activity of human alcohol dehydrogenase 4 (ADH4)?

Ivanildo Coutinho De Medeiros @Ivanildo_De_Medeiros

05 May 2014 1 5K Report

The enzyme has a high km for ethanol (about 30 mM).

6-4- Alcool déshydrogénase (ADH ; EC 1.1.1.1)

Ethanol +NAD+ Acétaldéhyde + NADH + H+

L’activité est mesurée, dans le tampon MR6, par l’augmentation de l’absorbance à 340 nm due à la formation de NADH (ε =6.22 103 M-1cm-1 ; Shimomura et Beevers, 1983a).

MR6: -Glycine………..100 mM; pH 9

-NAD+…………0.2 mM

-Ethanol………..100 mM

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