The enzyme has a high km for ethanol (about 30 mM).
6-4- Alcool déshydrogénase (ADH ; EC 1.1.1.1)
Ethanol +NAD+ Acétaldéhyde + NADH + H+
L’activité est mesurée, dans le tampon MR6, par l’augmentation de l’absorbance à 340 nm due à la formation de NADH (ε =6.22 103 M-1cm-1 ; Shimomura et Beevers, 1983a).
MR6: -Glycine………..100 mM; pH 9
-NAD+…………0.2 mM
-Ethanol………..100 mM
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