Whether "lattice parameter" and "lattice constant" are refering to same constants like a,b,c in crystals?

In crystal structures, the lattice constants are defined as "a", "b" and "c". In few literature(s), the "lattice parameter" is used. Are they same?. Is there any difference?.

27 January 2019 7,088 4 View

How useful is RIN value when you send out total RNA for miRNA sequencing?

I am extracting RNA from mouse tissue and would like to send the samples for sequencing, particularly looking for miRNAs in the samples. How useful and resourceful is the RIN value when the...

05 June 2018 3,300 3 View

Are there any specific microglial and astrocyte markers for primary cultures from postnatal mouse pups (P4-P7)?

Hi all. I am culturing primary astrocyte and microglia cells from postnatal mouse pups (P4-P7) and we are interested in looking at the gene expression specific for these cells types at this age....

06 March 2018 3,867 6 View

Can anyone share their experiences working with triple negative cell lines to screen for bioactivity?

Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...

09 February 2016 6,043 3 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

06 August 2015 2,210 5 View

How are collagens separated from their source?

How to isolate type-I collagen from its crude source (chicken waste). Generally people use hydrolysis by acid or alkali but I am looking for biological extraction methods.

30 January 2013 6,561 1 View

RT-qPCR Analysis

RT -qPCR analysis- I am new to qPCR, I have done the PCR and got cut off values (Ct) and I plotted a std graph using the ct values of my diluted and amplified cDNA samples. I want to analyse my...

31 October 2012 2,017 10 View

RT activity assay

HI I am working on porcine tissue and looking for porcine endogenous retrovirus and testing its infectivity on HEK cell line by RT activity assay. It has been suggested that we should check the RT...

14 November 2011 149 2 View

What is the difference between the use of Metasurface as substrate or superstrate for an antenna

If metasurface kept as a substrate or Superstrate to an antenna what are the differences what are the difference between the advantages and disadvantages between them

01 January 1970 6,441 6 View

Shared aperture antenna

In a substrate when the X band antenna elements and s band elements are placed but the ground plane is defected there is a big slot in the common ground plane. Can it be said as an shared aperture...

01 January 1970 521 9 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View