Whether "lattice parameter" and "lattice constant" are refering to same constants like a,b,c in crystals?

In crystal structures, the lattice constants are defined as "a", "b" and "c". In few literature(s), the "lattice parameter" is used. Are they same?. Is there any difference?.

27 January 2019 7,088 4 View

What is the minimum cDNA template amount to use for BioTaq Polymerase reaction?

I am doing a PCR with BioTaq polymerase enzyme from Bioline. I am not seeing any positive bands on my gel. Does anyone know the minimum amount of template to use for this enzyme reaction?

15 October 2018 1,316 3 View

How useful is RIN value when you send out total RNA for miRNA sequencing?

I am extracting RNA from mouse tissue and would like to send the samples for sequencing, particularly looking for miRNAs in the samples. How useful and resourceful is the RIN value when the...

05 June 2018 3,300 3 View

Are there any specific microglial and astrocyte markers for primary cultures from postnatal mouse pups (P4-P7)?

Hi all. I am culturing primary astrocyte and microglia cells from postnatal mouse pups (P4-P7) and we are interested in looking at the gene expression specific for these cells types at this age....

06 March 2018 3,867 6 View

Can anyone share their experiences working with triple negative cell lines to screen for bioactivity?

Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...

09 February 2016 6,043 3 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

06 August 2015 2,210 5 View

How are collagens separated from their source?

How to isolate type-I collagen from its crude source (chicken waste). Generally people use hydrolysis by acid or alkali but I am looking for biological extraction methods.

30 January 2013 6,561 1 View

RT-qPCR Analysis

RT -qPCR analysis- I am new to qPCR, I have done the PCR and got cut off values (Ct) and I plotted a std graph using the ct values of my diluted and amplified cDNA samples. I want to analyse my...

31 October 2012 2,017 10 View

RT activity assay

HI I am working on porcine tissue and looking for porcine endogenous retrovirus and testing its infectivity on HEK cell line by RT activity assay. It has been suggested that we should check the RT...

14 November 2011 149 2 View

What is the difference between the use of Metasurface as substrate or superstrate for an antenna

If metasurface kept as a substrate or Superstrate to an antenna what are the differences what are the difference between the advantages and disadvantages between them

01 January 1970 6,441 6 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

How can I convert permeability unit from barrer to m^2 for using COMSOL MultiPhysics?

I am looking for gas permeability of PDMS for simulation in COMSOL MultiPhysics and using creeping flow , fluid and matrix properties. The permeability unit in COMSOL is m^2 whereas I have the...

08 February 2021 4,305 2 View

Is it possible to highly selective remove a fused silica template from PDMS by laser-induced backside wet etching (LIBWE) method?

Hi everyone I'm trying to pattern circular cross-section microfluidic channels in PDMS. The template is an optical fiber with an axially non-uniform diameter. To the best of my knowledge, there...

22 January 2021 2,352 2 View

Is it possible to deposit very thin metal traces on a thin film of PDMS with long term durability?

Hi all researchers, I have been working on a research project where I need to fabricate very thin traces of the metal on a thin film of PDMS that can withstand large number of loading and...

06 January 2021 284 5 View

Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to slides?

Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to glass slides?.

04 January 2021 3,589 2 View

Vhb 4910 and carbon black as a electrode have some diference between carbon with pdms?

There is a dopant for the vhb 4910 actuator tapes that have acetylene black or carbon black which is carbon grease (carbon black + pdms) commercially, I am making a robotic actuator but I don't...

30 December 2020 3,144 1 View

How can I reduce the contraction between gold and PDMS?

I want to use gold electrode on the PDMS. We used an e-beam evaporator to deposit gold electrode on the PDMS using the recipe Ti 5nm, gold 80nm, right after using e-beam evaporator, there is no...

28 December 2020 8,607 3 View

Output signal can be detected at forward connection but no signal can be detected at reversed connection for my PVDF-based piezoelectric sensor, why?

20 December 2020 2,932 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View