What is the minimum cDNA template amount to use for BioTaq Polymerase reaction?

I am doing a PCR with BioTaq polymerase enzyme from Bioline. I am not seeing any positive bands on my gel. Does anyone know the minimum amount of template to use for this enzyme reaction?

15 October 2018 1,316 3 View

How useful is RIN value when you send out total RNA for miRNA sequencing?

I am extracting RNA from mouse tissue and would like to send the samples for sequencing, particularly looking for miRNAs in the samples. How useful and resourceful is the RIN value when the...

05 June 2018 3,300 3 View

Are there any specific microglial and astrocyte markers for primary cultures from postnatal mouse pups (P4-P7)?

Hi all. I am culturing primary astrocyte and microglia cells from postnatal mouse pups (P4-P7) and we are interested in looking at the gene expression specific for these cells types at this age....

06 March 2018 3,867 6 View

Can anyone share their experiences working with triple negative cell lines to screen for bioactivity?

Hi, I would like to hear suggestions about choosing cell lines for screening of small molecules. I understand that the common cell line used is MDAMB231. I also notice that use of MDAMB435 is...

09 February 2016 6,043 3 View

I am trying to run a flow cytometry experiment with MDAMB231 cells to do a cell cycle analysis. But why do I not see any cells when I run them?

06 August 2015 2,210 5 View

How are collagens separated from their source?

How to isolate type-I collagen from its crude source (chicken waste). Generally people use hydrolysis by acid or alkali but I am looking for biological extraction methods.

30 January 2013 6,561 1 View

RT-qPCR Analysis

RT -qPCR analysis- I am new to qPCR, I have done the PCR and got cut off values (Ct) and I plotted a std graph using the ct values of my diluted and amplified cDNA samples. I want to analyse my...

31 October 2012 2,017 10 View

RT activity assay

HI I am working on porcine tissue and looking for porcine endogenous retrovirus and testing its infectivity on HEK cell line by RT activity assay. It has been suggested that we should check the RT...

14 November 2011 149 2 View

What is the difference between the use of Metasurface as substrate or superstrate for an antenna

If metasurface kept as a substrate or Superstrate to an antenna what are the differences what are the difference between the advantages and disadvantages between them

01 January 1970 6,441 6 View

Shared aperture antenna

In a substrate when the X band antenna elements and s band elements are placed but the ground plane is defected there is a big slot in the common ground plane. Can it be said as an shared aperture...

01 January 1970 521 9 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View