Ketharini Senthilkumar

3 Questions 1 Answers 0 Followers

Questions related from Ketharini Senthilkumar

What is the minimum cDNA template amount to use for BioTaq Polymerase reaction?

I am doing a PCR with BioTaq polymerase enzyme from Bioline. I am not seeing any positive bands on my gel. Does anyone know the minimum amount of template to use for this enzyme reaction?

16 October 2018 1,430 3 View

How useful is RIN value when you send out total RNA for miRNA sequencing?

I am extracting RNA from mouse tissue and would like to send the samples for sequencing, particularly looking for miRNAs in the samples. How useful and resourceful is the RIN value when the...

06 June 2018 3,411 3 View

Are there any specific microglial and astrocyte markers for primary cultures from postnatal mouse pups (P4-P7)?

Hi all. I am culturing primary astrocyte and microglia cells from postnatal mouse pups (P4-P7) and we are interested in looking at the gene expression specific for these cells types at this age....

07 March 2018 3,986 6 View