Virtually unlimited, but that of course would depend on the particular conditions (amount of expressed Vir proteins, infection time, etc), but you can even introduce BACs through Agrobacterium-mediated transformation:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC38540/

Virtually unlimited, but that of course would depend on the particular conditions (amount of expressed Vir proteins, infection time, etc), but you can even introduce BACs through Agrobacterium-mediated transformation:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC38540/

Thanks Gustavo

Dear Can you please provide me in detail step by step protocol for Agrobacterium mediated transformation in Ganoderma lucidium. I will be highly obliged. Kind Regards 

dear Ali there are many protocol for gene transformation  to fungi but we only used C. Peter Romaine and Carl Schlagnhaufer method (Mushroom (Agaricus_bisporus)) for gene transformation into the common white mushroom.

https://www.researchgate.net/publication/6762326_Mushroom_(Agaricus_bisporus)

Article Mushroom (Agaricus bisporus)

Consider it unlimited although not true for A. tumefaciens and A. rhizogenes but transformation happens through 2 steps. First Agrobacterium mediated transformation into eukaryotic cell then its integration into genome. So the 2nd step would really be rate-limiting in context to integration into the host genome.

Yes the unlimited size is incorrect. i saw that the maximum size wich can be transformed by agrobactrium is about 30Kb