How to calibrate temperature on a Peqlab Primus 25?

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Focus on water use optimization or water resource optimization which one is effective way toward sustainability?

Water resources are limited and have been significantly reduced by pollution due to urbanization in recent decades. Therefore, water resource optimization only highlights the ways these resources...

27 July 2024 9,303 1 View

How to do Data Farming and Surrogate Model?

Dear Scholars How is it possible to define an algorithm for data augmentation and use a surrogate model? In particular, I want to use data farming to create artificial data instead of real data...

12 June 2024 2,484 4 View

Hi please how can i find quasi- experimental sample size calculator to compute the exact number ?

i need sample size calculator for quasi study design

02 June 2024 9,822 2 View

Hello drs Im asking about new topics in orthodontic?

hello drs can you help me finding new topics in orthodontic for PhD research, i preferred clinical topics specially using CBCT

30 April 2024 9,258 3 View

Is there a criterion for choosing the secondary outcome or the second dependent variable?

In an interventional study, is there a criterion for choosing a secondary outcome or a second dependent variable? Should these two variables in the study of medical sciences and especially...

26 April 2024 9,695 1 View

How long does e.coli survive on filter paper at room temperature?

I have been sent e.coli strains on filter paper and after 10 days I have not received them yet. I am wondering how long would the bacteria survive. Thank you in advance

21 April 2024 881 0 View

Re perform PCR reaction on the same first one for genotyping?

I performed genotyping analysis. Some samples give a result of one SNP but didnt give a result for another SNP. Note, these SNPs are performed at the same plate (same condition). Depending on the...

26 March 2024 7,013 0 View

Is the behavior of carbon steel (e.g., S275) under tension-compression tests symmetric?

Dear colleagues I am organizing tension tests on some existing steel elements (e.g., S275). I will extract the yielding strength and ultimate strength of the existing steel elements. I believe...

13 March 2024 2,035 0 View

Evaluation of seismic resilience of steel structures?

Question about how and types of methods to optimize steel structures for seismic resistance

29 February 2024 7,569 4 View

Why the CNN model is not trained?

I am trying to train a CNN model in Matlab to predict the mean value of a random vector (the Matlab code named Test_2 is attached). To further clarify, I am generating a random vector with 10...

13 January 2024 5,715 3 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

How do I correct that wrong author's profile in my publication?

I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...

28 July 2024 6,824 0 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

How to estimate a protien's size using Chimera or PyMol? how correct this estimated can be?

I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...

21 July 2024 5,732 3 View

How to ask to correct the Incorrect Claim regarding the writer of Article that I was involved in?

Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...

14 July 2024 9,579 2 View

V. Ravi

What is Peqlab Primus? If you could furnish more details it would be nice. In general any calibration is essential measuring the same quantity( measurand) by a reliable better instrument(reference ) and by the instrument under test.

Mohsen Navari

V. Ravi I am sorry, it is a thermal cycler by Peqlab.