Mohsen Ashrafi

6 Questions 35 Answers 0 Followers

Questions related from Mohsen Ashrafi

Standard error of differences or standard error of mean (Log) in qRT-PCR?

I would like to represent my qRT-PCR results in a bar plot but I am not sure to use SEofdiff or SEM as error bar?

09 July 2018 608 6 View

Could I use heat evaporation of methanol from my extracted metabolite instead of vacuum evaporation?

Hi everybody, I extracted my metabolite (a sample test) by methanol:water:chloroform (1:1:2) and after that polar phase was freeze-dried and NMR analysis was performed. one of my solvent...

20 May 2017 5,025 3 View

How could I group my data for analyzing in NormFinder algorithm in NormqPCR package of R?

Hi, I am trying to select most stable reference gene by use of gNorm and NormFinder algorithm of NormqPCR package. I had no problem when I use gNorm but when I want to use NormFinder it say "1....

08 April 2017 8,898 1 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

16 February 2017 3,707 3 View

Cell membrane stability over 100 percent?

Hi every one I calculated the CMS for my plants but after increase in severity of drought stress some of my plant species had cms about 200 or 300 percent. Why this state occurred? Is it...

11 October 2016 3,865 5 View

What is the maximum length of DNA which can be transformed by Agrobacterium tumefaciens?

Hi everybody. Does anybody know the maximum length of DNA (between left border and right border of plasmid) which can be transformed by Agrobacterium tumefaciens? thanks

18 September 2014 1,646 7 View