11 Questions 65 Answers 0 Followers
Questions related from Mohsen Ashrafi
I would like to represent my qRT-PCR results in a bar plot but I am not sure to use SEofdiff or SEM as error bar?
09 July 2018 680 6 View
Hi everybody, I extracted my metabolite (a sample test) by methanol:water:chloroform (1:1:2) and after that polar phase was freeze-dried and NMR analysis was performed. one of my solvent...
20 May 2017 5,087 3 View
Hi everybody, I want to identify and quantify plant metabolite by using 1D HNMR. I tested some R package like rNMR. This software is very user-friendly but it could not automatically identify...
25 April 2017 4,813 9 View
Hi, I am trying to select most stable reference gene by use of gNorm and NormFinder algorithm of NormqPCR package. I had no problem when I use gNorm but when I want to use NormFinder it say "1....
08 April 2017 8,971 1 View
hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...
16 February 2017 3,796 3 View
Hi every one I calculated the CMS for my plants but after increase in severity of drought stress some of my plant species had cms about 200 or 300 percent. Why this state occurred? Is it...
11 October 2016 3,912 5 View
I want to use NMR for plant metabolomics but I confused to select NMR method.We have NMR (450 mhz, 1H, C, cosy). according to mentioned NMR instrument, Which of these methods is more effective for...
09 September 2016 1,445 11 View
Hello every one I am trying to draw a box plot in R but only half of the my X labels are shown in it. Its picture is attached. how can I fix it? Thanks a lot
25 May 2016 7,848 14 View
Hello every one, I am looking for a protocol for Extraction of plant leaf metabolite (for metabolite profiling) (Thyme species) to analyse it by H-NMR. I have seen some protocol in the internet...
23 August 2015 3,049 10 View
I want to synthesise a 8500bp cDNA but its RNA has multiple loops (overall deltaG= -960 only predicted for 3500bp in clc mainworkbench). How can i synthesize it? Do loops interrupt cDNA synthesis?...
16 February 2015 9,117 3 View
Hi everybody. Does anybody know the maximum length of DNA (between left border and right border of plasmid) which can be transformed by Agrobacterium tumefaciens? thanks
18 September 2014 1,696 7 View