Mohsen Ashrafi

13 Questions 71 Answers 0 Followers

Questions related from Mohsen Ashrafi

Standard error of differences or standard error of mean (Log) in qRT-PCR?

I would like to represent my qRT-PCR results in a bar plot but I am not sure to use SEofdiff or SEM as error bar?

09 July 2018 682 6 View

Could I use heat evaporation of methanol from my extracted metabolite instead of vacuum evaporation?

Hi everybody, I extracted my metabolite (a sample test) by methanol:water:chloroform (1:1:2) and after that polar phase was freeze-dried and NMR analysis was performed. one of my solvent...

20 May 2017 5,091 3 View

Could anybody suggest a free software identification and quantification of 1D HNMR?

Hi everybody, I want to identify and quantify plant metabolite by using 1D HNMR. I tested some R package like rNMR. This software is very user-friendly but it could not automatically identify...

25 April 2017 4,817 9 View

How could I group my data for analyzing in NormFinder algorithm in NormqPCR package of R?

Hi, I am trying to select most stable reference gene by use of gNorm and NormFinder algorithm of NormqPCR package. I had no problem when I use gNorm but when I want to use NormFinder it say "1....

08 April 2017 8,979 1 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

16 February 2017 3,803 3 View

Cell membrane stability over 100 percent?

Hi every one I calculated the CMS for my plants but after increase in severity of drought stress some of my plant species had cms about 200 or 300 percent. Why this state occurred? Is it...

11 October 2016 3,917 5 View

Which method I must use for NMR based plant metabolomics?

I want to use NMR for plant metabolomics but I confused to select NMR method.We have NMR (450 mhz, 1H, C, cosy). according to mentioned NMR instrument, Which of these methods is more effective for...

09 September 2016 1,449 11 View

How can I draw a plot with custom Y-axis in R?

Hi every body I want to draw a bisection Y-axis plot which scaled from 0 to 100. At first section (1 to 30) it would be ranged by 2 (2,4,6,...,30)and after that it would be ranged by 10...

02 August 2016 4,260 4 View

How cloud I have all X label in my box plot?

Hello every one I am trying to draw a box plot in R but only half of the my X labels are shown in it. Its picture is attached. how can I fix it? Thanks a lot

25 May 2016 7,859 14 View

Does anybody have a better protocol for plant metabolite extraction?

Hello every one, I am looking for a protocol for Extraction of plant leaf metabolite (for metabolite profiling) (Thyme species) to analyse it by H-NMR. I have seen some protocol in the internet...

23 August 2015 3,053 10 View

Does anybody have a good protocol for long cDNA synthesis?

I want to synthesise a 8500bp cDNA but its RNA has multiple loops (overall deltaG= -960 only predicted for 3500bp in clc mainworkbench). How can i synthesize it? Do loops interrupt cDNA synthesis?...

16 February 2015 9,121 3 View

Does someone know about identification of genes involved in a bio synthetic pathway?

i want to identify genes involved in a biosynthesis pathway and need help to choose best and low equipment required method to do this. thanks for your helps

02 October 2014 6,831 10 View

What is the maximum length of DNA which can be transformed by Agrobacterium tumefaciens?

Hi everybody. Does anybody know the maximum length of DNA (between left border and right border of plasmid) which can be transformed by Agrobacterium tumefaciens? thanks

18 September 2014 1,699 7 View