What is the major difference in supporting cellular functions between GelMA gels and microbial transglutaminase crosslinked Gelatin Hydrogels?

Budharaju Harshavardhan @Budharaju-Harshavardhan

30 March 2021 0 8K Report

Based on the literature GelMA hydrogels are supporting cellular functions and maintaining cell morphology in a better way compared to transglutaminase crosslinked gelatin hydrogels. Is there any specific reason for that?

Badges
Science method

More Budharaju Harshavardhan's questions See All

Reaction Equilibrium attaining at low temparature than literature in aspen plus?

I am modelling the calcination process in RGibbs reactor but the reaction occurs at low temparature 800 °C instead 860. and how can i solve this issue without changing the reactor. I have tried...

30 March 2023 4,951 1 View

How to plot theoretical radiation pattern of an couple dipole antenna with following parameters?

Frequency 980.0MHz, Active Dipole Length 0.155m, Passive Dipol Length 0.123m Distance between dipoles0.045m

01 August 2020 1,081 2 View

How to extract reaction forces in LS-Dyna?

Hi, I want to extract reaction or nodal forces from the nodes that are constrained to form a rigid body using *Constrained_Nodal_Rigid_Body. Currently, in ASCII, nodfor and nodout show that...

26 January 2020 1,386 5 View

How to use NURBS elements in LS-Dyna?

Hi, I have been unsuccessful in using *ELEMENT_SHELL_NURBS_PATCH keyword in LS-Dyna. The job manager terminates without mentioning any errors in message file. I guess I am not using the keyword...

01 January 2020 6,440 1 View

Textile conductive material definition in HFSS

Hi, I am designing a textile antenna, for this purpose I need to define a Shieldit Fabric conducting material with a surface resistivity of 0.01-ohm meter and conductivity of 100 S/m.

06 August 2017 1,121 1 View

Are there any studies on static inward inflation of nonlinear elastic membranes?

By inward inflation, I mean vacuum inside the membrane (such as a cylinder with closed ends) leading to its crushing by atmospheric pressure.

23 June 2017 2,932 3 View

What is the radiation box size in HFSS for Very compact patch antennas fed by CPW ?

Dear friends... I have designed several very compact antennas from reputed journals in HFSS. But I am getting the negative gains for low-frequency band (3GHz to 4 GHz). I could not find the...

09 May 2017 1,788 17 View

How to find the group delay of an UWB antenna in HFSS ?

Hi, please provide the necessary steps involved in plotting the group delay vs frequency for an UWB in HFSS,

30 March 2017 542 8 View

UWB antenna?

Currently, I am designing a UWB microstrip antenna based on IEEE paper, I could not reproduce the result (s11) given in the paper. I am attaching the IEEE paper and my design, please provide...

08 November 2016 561 5 View

Cpw fed UWB patch antenna boundary condition problem?

hello currently i am designing a cpw fed UWB antenna in hfss.while checking the boundary conditions warning shown in the below image occurred, can you please provide solution to...

26 September 2016 3,344 4 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

28 July 2024 2,794 6 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

Concentration of gelatin as brain model ?

I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.

19 July 2024 3,095 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

immunofluorescence, False Positive Nuclear Staining in Hydrogel-Embedded Breast Cells?

I have primary breast cells embedded in the hydrogel. When stained with EpCAM without the hydrogel, the staining was correctly localized to the membrane as expected! However, after embedding in...

28 June 2024 353 1 View