since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf?
thanks a lot
Endotelial cells are negative for CD73 (and mesenchymal cell are positive).
There is a more complete discussion in this other question:
https://www.researchgate.net/post/Which_panel_of_antibodies_for_detection_of_bone_marrow_stromal_and_endothelial_cells_by_flow
However take into account that in Mesenchynal cells culture conditions, even if there were some endothelial cells, they will eventually be lost through passages.
MSC has a spindle shape and high proliferation rate
What challenges do you think businesses or entities face in implementing these strategies? Thanks
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