How can I magnify an image without changing the dimension in matlab?

Hi everyone. For the last part of my project I have to see how does my program respond to a magnified image. The magnification has to be a part of the code . I've tried imresize() , it will be...

10 February 2021 8,279 10 View

How to know initial values for non-linear regression?

Does anyone know a reliable methodology to estimate the initial values of theta1 (or A) and theta2 (or B) in a non-linear model? For example, a = (A+Bx/hc)(hc/x-Eg) How to reliably estimate the...

17 January 2021 1,327 1 View

How can i get AMPS-1D resources?

I am trying to model a photovoltaic device using AMPS-1D software. But once I am submitting it to queue, it remains there for infinite amount of time without giving the result. I couldnt...

11 January 2021 1,829 1 View

How can I omit some special part of an image in matlab?

Hi I'm trying to edit my input image which is a simple Persian letter. I want to omit the dots of the letter. I can do it with Photoshop of course, but it would be more technical if I could do it...

14 December 2020 201 5 View

How can I produce hyper immune mouse against human FZD7?

My thesis about the production of mouse anti-human FZD-7 monoclonal antibody (against the 33-185 AA of FZD7) by the hybridoma method. I have a problem with mouse immunization by FZD7. In ELISA the...

10 October 2020 3,741 2 View

How can I design Vanderlugt filter in Matlab?

Hi everyone I need some help in designing Vanderlugt filter in Matlab. I want to design this filter and later use it in a 4f optical correlator. As I tested my designed filter and I'm not sure...

19 September 2020 8,662 7 View

What is the best way to show my live data as an online dashboard using Python?

Hello, I have plenty of data coming from my sensors every minute and I analyze them in Python. I want to make a realtime online dashboard to make a nice representation of my data. My ultimate...

28 May 2020 1,149 3 View

What is the best practice to implement machine learning on real world sensor data?

Hello, I have a few sensors in a home. I am going to continuously receive data fro these sensors, and do Machine Learning (in Python. Keras) on stored data every 24 hours. One possibility to do...

20 May 2020 9,285 7 View

Is there an open-source IoT platform to do online Machine Learning for free?

I am going to connect my sensors to an IoT platform to receive their data and store. Then I need to do machine learning predictions (as Python script) every 24 hours to do predictions for the next...

20 May 2020 590 5 View

How to check the homogeneity of variance in large sample size with 6000 variables?

I use R to do univariate regressions for a large data set consisting of 6000 variables. I would like to know whether checking the homogeneity of variance is necessary for large sample or not....

20 February 2020 5,989 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View