Here's the NOAEL 

1.8 mg FB1/kg bw/day (Voss et al, 1995)

According to IARC Monographs (Vol.) 82 -

"The single-dose LD50 of fumonisin B1 is unknown. Single gavage doses of 50, 100 and 200 mg/kg bw fumonisin B1 significantly inhibited hepatocyte proliferation when given to male Fischer rats six hours after partial hepatectomy (Gelderblom et al., 1994). In male Sprague-Dawley rats, intravenous injection of fumonisin B1 at 1.25 mg/kg bw resulted in histological changes in the outer medulla of the kidney, with an increased number of mitotic figures and apoptosis followed by severe nephrosis (Lim et al., 1996)." 

The exact procedure for FB extraction would be dependent on the tissue, but most involve extraction with Formic acid acidified acetonitrile-water extraction. 

Goodluck!

A.

NOAEL from two studies (mouse), expressed as mg FB1/kg b.w./day:

1) Subchronic study

1.8 mg FB1/kg bw/day (Voss et al, 1995)

Voss, K.A., Chamberlain, W.J., Bacon, C.W., Herbert, R.A., Walters, D.B. and Norred, W.P. 1995. Subchronic feeding study of the mycotoxin fumonisin B1 in B6C3F1 mice and Fischer 344 rats. Fundam Appl Toxicol 24(1):102-10.

2) Chronic study

0.6 mg FB1/kg bw/day (US-NTP, 1999)

US-NTP (United States National Toxicology Program), 1999. Toxicology and carcinogenesis studies of fumonisin B1 (CAS no 116355-83-0) in F344/N Rats and B6C3F Mice (Feed studies). NTP Technical Report TR 496; NIH Publication No 99-3955. U.S. Department of Health and Human Services, Research Triangle Park, North Carolina, USA

B.

Lawrence JF, Niedzwiadek B, Scott PM. Effect of temperature and solvent composition on extraction of fumonisins B1 and B2 from corn products. J AOAC Int. 2000; 83(3):604-11.

Abstract

Fumonisins B1 and B2 were extracted from naturally contaminated corn products by using different extraction solvent compositions (methanol-water, acetonitrile-methanol-water, ethanol-water, and 100% water) and a range of temperatures from ambient to 150 degrees C. Ground samples of several corn products and 1 rice sample were mixed with an adsorbent material (Hydromatrix), and the fumonisins were extracted in 2 sequential 5 min static extractions at various temperatures. The combined extracts were cleaned up and analyzed by reversed-phase liquid chromatography with fluorescence detection after o-phthaldialdehyde-mercaptoethanol derivatization. The results showed a clear influence of temperature and solvent composition on recovery of fumonisins from some matrixes. With acetonitrile-methanol-water (1 + 1 + 2) the quantity of fumonisins extracted from naturally contaminated taco shells almost tripled in going from 23 degrees to 80 degrees C, and increased by another 30% when ethanol-water (3 + 7) was used as extraction solvent at 80 degrees C. Similar results were obtained with nacho chips. These effects were less pronounced with cornmeal, and small differences due to temperature and solvent composition were observed for corn flakes and rice. The ethanol-water extraction solvent combinations were specifically evaluated in an effort to use the cheapest, least toxic, and most environmentally friendly solvents for organic residue analysis. At 80 degrees C, ethanol-water combinations performed equally or better than methanol-water (8 + 2) or acetonitrile-methanol-water (1 + 1 + 2), combinations which are commonly used for fumonisin extractions. Even 100% water was successful for extracting fumonisins from the products, except for rice. However, increased amounts of water created technical problems and required an increased amount of Hydromatrix in the samples prior to extraction.

Best regards

Vladimir

Thanks for helping me

according to voss et al 1995 (1.5-1.8) mg / kg body weight