I need help knowing that how many sperms are needed in order to perform DNA fragmentation test of human sperm?
if it is possible provide complementary references with your answer
Thanks in advance
There is no cut off value for this assessment. However, it is difficult to perform the test with severe oligozoospermic samples. If the sperm count is very low you can centrifuge the sample and perform the test to pelleted sperm. According to my point of view, performing the test for severe oligozoospermic samples has a limited value. Because those samples would already subfertile due number of other abnormalities.
about some microletrr for stain on slide and also some microleter for compute the concentration on hemocytometer respect to red blood
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