How can I preserve my NGF media?

02 March 2014 8,739 3 View

How can I separate the cells of my PC-12 cell line?

I have worked on a PC-12 cell line and I have a hard time transferring the cells. The problem is: it's very difficult to separate the cells into single cells after centrifuging, even if I mixed...

02 March 2014 4,388 9 View

How to analyse data by SPSS?

I want to know what the difference is between one-way ANOVA and T-test. How do I draw a significant graph with the P data from the result of ANOVA or T-test?

11 December 2013 787 2 View

Can someone recommend an anti-body for tyrosinase inhibition?

I am working in whitening-skin experiments. Could someone tell me what anti-body use for Tyrosinase inhibition and melanin synthesis inhibition in western-blot?

06 July 2013 1,174 0 View

Mushroom genomic DNA

Please teach me what kind of DNA marker use for mushroom genomic DNA agarose gel electrophoresis.

04 May 2013 7,353 9 View

Antibody for Weston-blot

Can I use several primary anti-body before secondary anti-body?

04 May 2013 2,677 11 View

What is different between right hind paw and left hind paw of Rat using an anti-inflammatory activity experiment?

I am doing in vivo anti-inflammatory activity and I do not understand why every one do that experiment use only right hind paw of rat. Some one can teach me what is the different?

03 April 2013 7,662 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View