How can I preserve my NGF media?

02 March 2014 8,739 3 View

How can I separate the cells of my PC-12 cell line?

I have worked on a PC-12 cell line and I have a hard time transferring the cells. The problem is: it's very difficult to separate the cells into single cells after centrifuging, even if I mixed...

02 March 2014 4,388 9 View

How to analyse data by SPSS?

I want to know what the difference is between one-way ANOVA and T-test. How do I draw a significant graph with the P data from the result of ANOVA or T-test?

11 December 2013 787 2 View

Can someone recommend an anti-body for tyrosinase inhibition?

I am working in whitening-skin experiments. Could someone tell me what anti-body use for Tyrosinase inhibition and melanin synthesis inhibition in western-blot?

06 July 2013 1,174 0 View

What is the inhibition of collagenase and calastase

I am doing an experiment about the inhibition of collagenase and calastase activity. Does anyone know what is the inhibitor of those enzymes? Thank for you time!

05 June 2013 4,656 1 View

Mushroom genomic DNA

Please teach me what kind of DNA marker use for mushroom genomic DNA agarose gel electrophoresis.

04 May 2013 7,353 9 View

Antibody for Weston-blot

Can I use several primary anti-body before secondary anti-body?

04 May 2013 2,677 11 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View