I am working in whitening-skin experiments. Could someone tell me what anti-body use for Tyrosinase inhibition and melanin synthesis inhibition in western-blot?
I have made media content 50 ng/mL of NGF (nerve growth factor). Can I make a stock at -20°C for few days? If it is possible, how long can I keep? Thank for any help.
02 March 2014 8,843 3 View
I have worked on a PC-12 cell line and I have a hard time transferring the cells. The problem is: it's very difficult to separate the cells into single cells after centrifuging, even if I mixed...
02 March 2014 4,496 9 View
I want to know what the difference is between one-way ANOVA and T-test. How do I draw a significant graph with the P data from the result of ANOVA or T-test?
11 December 2013 897 2 View
I am doing an experiment about the inhibition of collagenase and calastase activity. Does anyone know what is the inhibitor of those enzymes? Thank for you time!
05 June 2013 4,780 1 View
Please teach me what kind of DNA marker use for mushroom genomic DNA agarose gel electrophoresis.
04 May 2013 7,457 9 View
Can I use several primary anti-body before secondary anti-body?
04 May 2013 2,776 11 View
I am doing in vivo anti-inflammatory activity and I do not understand why every one do that experiment use only right hind paw of rat. Some one can teach me what is the different?
03 April 2013 7,781 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View