How can I preserve my NGF media?

02 March 2014 8,739 3 View

How can I separate the cells of my PC-12 cell line?

I have worked on a PC-12 cell line and I have a hard time transferring the cells. The problem is: it's very difficult to separate the cells into single cells after centrifuging, even if I mixed...

02 March 2014 4,388 9 View

How to analyse data by SPSS?

I want to know what the difference is between one-way ANOVA and T-test. How do I draw a significant graph with the P data from the result of ANOVA or T-test?

11 December 2013 787 2 View

What is the inhibition of collagenase and calastase

I am doing an experiment about the inhibition of collagenase and calastase activity. Does anyone know what is the inhibitor of those enzymes? Thank for you time!

05 June 2013 4,656 1 View

Mushroom genomic DNA

Please teach me what kind of DNA marker use for mushroom genomic DNA agarose gel electrophoresis.

04 May 2013 7,353 9 View

Antibody for Weston-blot

Can I use several primary anti-body before secondary anti-body?

04 May 2013 2,677 11 View

What is different between right hind paw and left hind paw of Rat using an anti-inflammatory activity experiment?

I am doing in vivo anti-inflammatory activity and I do not understand why every one do that experiment use only right hind paw of rat. Some one can teach me what is the different?

03 April 2013 7,662 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View