Any one interested in publishing papers in fluid dynamics?

willing to publish a paper

03 June 2019 5,402 2 View

How can we perform the urease and nitrate tests for an alkalibacterium that grows only above pH 8?

The protocols available are optimised at ph 6.8 to 7.

07 September 2017 8,796 2 View

EMSA kd interpretation?

I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...

24 September 2015 5,366 3 View

Are phosphorylated proteins of insulin signalling pathway very labile?

I have run western blots and have got clean bands for AKT, phospho Akts and others but I am not able to get clear bands on repeating the western from the same samples around 3 weeks later. My...

30 December 2014 4,990 5 View

How do you get a band in a western blot for Insulin receptor beta and phosphorylated insulin receptor beta?

I have done my western blotting using C2C12 cell lysate sample but I am not able to get the band for the above. I am using Santa Cruz antibodies for the insulin receptor beta subunit and its...

14 December 2014 8,237 4 View

How can I find the transfection efficiency when we use commercially available non-fluorescent siRNA for transfection?

I have used commercially control and target siRNA from SantaCruz to perform transfection in C2C12 and HSMM. I performed western blot and the target protein was not expressed while the controls...

24 November 2014 1,757 15 View

What is the housekeeping protein for C2C12?

Housekeeping protein for mice myoblast cell lines

18 November 2014 2,251 3 View

How to avoid non specific band in western blot ?

i am using a polyclonal rabbit primary for detecting a 50KDa protein but keep getting nonspecific band around 30KDa and few others. I block using 5% non fat milk for one hour sample preparation by...

28 October 2014 3,198 7 View

Is immunoprecipitation required for western blot analysis of both phosphorylated and nonphosphorylated insulin receptor beta and IRS ?

28 September 2014 8,007 5 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

In your experience what is the difference between G.652D and G.655D optical fiber. In field and in lab?

In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...

11 February 2021 4,006 8 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View