What is the incubation time required to use insulin as an inducer to study its signalling pathway in skeletal muscle (C2C12 and HSMM)?

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Urbach energy calculation directly from Tauc's plot?

Can we find Urbach energy from the Tauc's plot itself? That means, by extrapolating the urbach tail to x axis of Tauc's plot..

26 July 2023 423 0 View

RNA markers for cell fractionation?

Which can be ideal RNA markers for cell fractionation, as in for nuclear fraction and cytoplasmic one? I need to work on lncRNA localization in the different cell fractions. Thanks in Advance!!!

28 February 2022 6,846 1 View

Is their any RNase inhibitor that can be used along with the fractionation kit buffers which need not need autoclaving or pre-heating?

Hi, I am trying to use a cytoplasmic nuclear fractionation kit which is employed for protein analysis. However, I need to analyze the RNA as well using the kit and was looking for any RNase...

21 February 2022 5,760 1 View

Any one interested in publishing papers in fluid dynamics?

willing to publish a paper

03 June 2019 5,607 2 View

Control of STATCOM using dSPACE?

Why the output voltage of inverter is zero eventhough the pulses are coming correctly from dSPACE controller.

24 April 2018 4,792 5 View

How can we perform the urease and nitrate tests for an alkalibacterium that grows only above pH 8?

The protocols available are optimised at ph 6.8 to 7.

07 September 2017 8,939 2 View

EMSA kd interpretation?

I have done F- EMSA and used 200nm of my DNA and titrated it with different concentration of my repressor protein. I found the kd to be 1.6 micro molar and I used prism sofware to fit my data with...

24 September 2015 5,477 3 View

Any suggestions on protein aggregates during buffer exchange in size exclusion?

Hi fellow researchers, am an newbie in protein purification and hence this question. I am working on a novel protein whose size is 17kda.it does not have cysteine. It's PI is around 7.83. I did a...

20 June 2015 2,793 5 View

What is the significance of serum starvation when studying molecular signalling pathways especially Insulin signalling?

Why do researchers serum starve the cells before studying the molecular event in a cell line. I am planning to look at the Insulin signalling events in skeletal muscle cell lines, should I serum...

27 February 2015 7,115 5 View

Please can anyone tell how much PEDF is secreted from human skeletal muscles roughly?

Do anyone have an idea about PEDF secretion from skeletal muscle.

11 February 2015 7,695 1 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

Does EMG show muscle spasms?

Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?

20 July 2024 5,951 1 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View