25 July 2024 1 3K Report

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

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Procedure of Ammonium sulfate precipitation of ACE2 in Calu-3 cells?

Hello, I am trying to find a protocol that looks at the extraction and purification of ACE2 inside the cell. We have the protocol for the affinity chromatography, but we are having trouble...

23 May 2024 4,342 1 View

Can anyone identify these Cumaceans?

Hi everyone, I've been struggling to identify the family these guys belong to. Does anyone have any ideas? Taken from NE Atlantic 2200m depth.

23 April 2024 1,282 4 View

When washing demineralized fish scales, should I be concerned when there are string like substances (most probably fibers) are present?

My study is about synthesizing fish scales into chitosan and I am currently washing my demineralized fish scales. My sample fish scales were size reduced beforehand and when 1% HCl was added, it...

16 April 2024 2,129 0 View

In assessing the viability of using lithium heparin from gel separatory serum, what other tests can be added as parameters to further the study?

In the present day, countless tests are being run in the clinical laboratory. In the Journal of Oguzhan Zengi entitled, "The Transition from Gel Separatory Tubes to Lithium Heparin Gel Tubes in...

10 April 2024 3,541 1 View

How to make a thesis introduction?

how to start my thesis introduction

24 March 2024 2,366 1 View

What are some economical methods which can be employed by tertiary hospitals to develop & utilize regional antibiograms in the Philippines?

In the Philippines, national databases on antibiotic resistance are noted through the Department of Health (DOH) Antimicrobial Resistance Surveillance Program (ARSP). However, community or...

29 February 2024 4,600 1 View

What's wrong with Oil red O staining of the liver slices?

Hi everyone, the attached picture is oil red o staining of mouse liver slices after GAN diet, which was to induce NASH. I am wondering what are those round empty space with the clear boundary? Are...

27 February 2024 5,743 0 View

Fixed brain in 30% sucrose solution froze at 4°C?

I've been trying to figure out what happened but it seems I am the only one this has happened to? Processing: - transcardial perfusion with 1x PBS followed by 4% PFA - post-fix brain in 4% PFA...

12 February 2024 5,963 2 View

Sharing link to ResearchGate on LinkedIn?

is It possible to share my ResearchGate profile on LinkedIn so I only need to update one of them? Thanks!

28 October 2023 4,675 0 View

What is the best way to get clean the stacking gel of a western blot gel after taking out the comb?

Whenever I do a western blot, when the comb comes out of the stacking gel, there are little bits of gel stuck in the wells and some bubbles. What is the best way to get rid of the debris and bubbles?

19 October 2023 2,405 2 View

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Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

How can I calculate formation energy using VASP-Ab-initio?

I would like to calculate the formation energy of P2-Na0.67Fe0.5Mn0.5O2 based on DFT, what should I do step by step. Any help would be appreciated. Thanks.

29 July 2024 8,248 2 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

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