25 July 2024 1 3K Report

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

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Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

Bacterial Contamination in Laminar Flow Hood?

Hi there! Brace yourself, this is going to be long winded and contains a year's worth of frustrations. I culture moss, and for the last year, I have been fighting a gram negative bacterial...

29 August 2020 6,492 2 View

Has anyone used Python for qualitative research analysis?

I am looking into the benefits of using programming skills for data coding and analysis. This stems from the need for highly technical skills when considering roles outside academia. If you have...

23 June 2020 9,142 1 View

Pittsburgh Sleep Quality Index Scoring Synthax on Excel?

Hi, Just checking if anyone has an Excel scoring synthax for the Pittsburgh Sleep Quality Index that they would be happy to share Thanks Kate

24 March 2020 4,047 2 View

Can I Combine Data and Tree Information into One Tree in Mesquite?

Is it possible to enter two different types of information into Mesquite? I have two sets of species data: the divergence times (in millions of years) i.e. how old each species is and the...

22 March 2020 6,304 3 View

Please how do i follow researchers on a particular field?

For instance, I'm writing on economic recession, I want to follow foreign researchers so as to be guided by their works.

27 January 2020 608 3 View

Can phenomenography be used even with limited variation in the data collected?

I am currently conducting a study and have collected interview data from students on their perspectives and experiences. I had anticipated that these experiences would be different based on their...

06 January 2020 9,667 2 View

How long can tissue homogenized in Trizol be store at -30c for?

Our lab mistakenly stored homogenized tissue samples in Trizol at -30 for an extended period of time (over a year). Should we toss these samples or should they be moved to -80? We don't plan on...

18 September 2019 1,216 1 View

Identification of conserved transcription factor binding sites across species?

Hi all, I'm trying to use PWMs for a transcription factor of interest to identify conserved binding sites in upstream promoters (up to 5 kb from TSS), across several vertebrate species. My model...

22 May 2019 1,066 1 View

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Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

Measurement of water retention in organic substrates without a pressure plate?

Hello, I'm trying to measure the physical and hydrological properties of organic substrate media. Currently, I'm referring The book Soil sampling and Methods of Analysis by Carter and Gregorich....

09 February 2021 3,233 8 View

Any model suggestion for the Electronic Health Records dataset ?

I have sequentially measured the time-series Electronic Health Record patient dataset. This is 7 years of research and each patient's blood pressure, Hba1c, LDL, Hb, Baseline Age,...were measured...

09 February 2021 4,933 8 View

Does Gaussian 09W software take too long to give a result if we have a large chemical structure ?

DFT - gaussviev - gaussian09W - HOMO - LUMO

08 February 2021 6,012 5 View

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