What are the very crucial steps in control of plant gene expression and what is the importance of timing (mRNA transport control, mRNA translation control, mRNA & protein stability & degradation control)
:D I'm interesting on learning & studying;
if there is no timing ???
i feel there may be lagging of collapsing of biochemical pathways
Polymerase preloading may have timing and coordination
sometimes this will affect on where one Gene restrict the action of other genes to specific regions / pathways of the organism
May be some pathways share same sequences / same biochemical in their pathway, so one should wait till that use :)
Answer received from : Kapila Gunasekera
I think It mainly depends on the functions of each protein. If you measure on and off time over period for each mRNA and protein you could group them into several groups. Probably house keeping gene would show you the same oscillation pattern. The proteins with long half life would show you a different oscillation pattern. you would also notice that oscillation pattern of some of the genes are cell cycle dependent. If the cells are under stress situations then you would see a global change in oscillation patterns.
So the question should be based on a single gene or a group of genes like house keeping genes etc. and this could be measured using number of approaches.
Did I answer your question.
Exactly this is what i wanted to know, thank you sir :)
Answer received from : Kapila Gunasekera
the available approaches to measure>>>>>
This would be a good starting point
http://genomebiology.com/2011/12/3/107
RNAfish is another approach. You will be able to read technical details of the products available here.
http://www.lifetechnologies.com/ch/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fluorescence-in-situ-hybridization-rna-fish.html
Here are some more stuff to read
http://www.ncbi.nlm.nih.gov/pubmed/17641652
http://www.plantmethods.com/content/2/1/18
http://www.illuminatedcell.com/imaging-rna.html
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0048736
http://www.plantphysiol.org/content/147/4/1469
Hope these help you.
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