How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

What molecules bind to dsDNA?

Hello, there are some molecules that bind to double-stranded DNA; for instance, SYBR green and some antibodies. Are there more examples? Thank you

25 January 2021 7,595 4 View

What are best conditions for in vitro hybridization?

Hello, I would like to hybridize a probe and an anti-probe (ssDNA oligonucleotides) in a reaction vessel. What would be the best buffer to use? and the temperature of annealing? Thank you

06 December 2020 4,121 0 View

Which is the best statistical approach to an experimental measurement?

Hi everyone, my question is the following. I have to perform an experiment with the aim of finding a correlation between a quantity X (e. g. temperature) and a quantity Y (depending on X). Which...

06 October 2020 3,911 6 View

How to activate polystyrene with carboxyl group?

Is there a simple (that is one that requires few and not very exotic reagents) chemical reaction to "activate" or "functionalize" the polystyrene of a simple microtitre plate? In other words, I...

06 September 2020 233 3 View

What is the stability of EDAC in aqueous solution?

06 September 2020 4,305 3 View

What test to compare shannon index results?

Hello, I have obtained the following Shannon indices from a trio of samples: 27.73 (healthy tissues), 24.55 (primary colon tumor), and 1.20 (metastatic tissues). The indices have been obtained...

30 August 2020 5,543 1 View

What is the concentration of acid for an ELISA stop solution?

Hello, what is the correct molarity of a stop solution for a peroxidase using o-Phenylenediamine dihydrochloride (OPD)? I am getting all sorts of molarities, from fractional up to 3 M according to...

01 July 2020 3,729 1 View

Is there a database for bacterial features?

Hello, is there a repository of the basic characteristics of bacteria, such as maximum growth rate? In the works related to the modelling of bacterial growth, the parameters requested are always...

25 February 2020 5,498 2 View

Can the PCR fail to amplify high loads of target?

Hello, I did the PCR (long-range, target ~5 Kb) that is shown in the attached file. The lanes are: 1. ladder 2. target (plasmid purified from E. coli after transfection), 50 ng/uL corresponding to...

18 February 2020 6,863 3 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

What is the best way to isolate multiple Salmonella serovars from a single sample?

I am trying to isolate multiple Salmonella serovars from a single food sample. I started by incubating the sample in a non-selective BPW enrichment and from there used multiple selective...

19 January 2021 8,365 4 View

What are the antimicrobial categories and agents that are currently considered to define a MDR, XDR and PDR Salmonella?

What antimicrobial categories and agents are currently considered to define non-typhoid Salmonella as multidrug resistant (MDR), extremely / extensively drug resistant (XDR) or pandrug resistant...

19 November 2020 755 3 View

How to knock out the gene of multidrug-resistant salmonella typhimurium?

During the knockout process, I found that my Salmonella was resistant to multiple antibiotics and could not screen successfully imported transgenes. Moreover, my bacteria seems to be very...

12 September 2020 6,683 1 View

Determination of Lipid A in Salmonella?

Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....

26 August 2020 9,455 1 View

Can any one tell me thet either anti salmonella group o antibodies and anti salmonella h-d antibody conjugates/binds with E coli and Staph?

i have been working on anti salmonella group o and anti salmonella h-d antibodies, i need to know that is there any interaction between these salmonellic antibodies with E coli and staph? As...

11 December 2019 8,579 1 View

Can any one tell me thet either anti salmonella group o antibodies and anti salmonella h-d antibody conjugates/binds with E coli and Staph?

i have been working on anti salmonella group o and anti salmonella h-d antibodies, i need to know that is there any interaction between these salmonellic antibodies with E coli and staph? As these...

11 December 2019 1,794 1 View

Can any one tell me thet either anti salmonella group o antibodies and anti salmonella h-d antibody conjugates/binds with E coli and Staph?

i have been working on anti salmonella group o and anti salmonella h-d antibodies, i need to know that is there any interaction between these salmonellic antibodies with E coli and staph? As...

09 December 2019 901 1 View

What is the appearance of Listeria monocytogenes on other selective media (besides ALOA)?

Specifically for Rapid EC, Rapid Salmonella, Columbia blood agar, MYP, TSA, and XLD. Can it still grow? If so, what color and zone of inhibition?

10 November 2019 202 3 View