How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

What is the best way to isolate multiple Salmonella serovars from a single sample?

I am trying to isolate multiple Salmonella serovars from a single food sample. I started by incubating the sample in a non-selective BPW enrichment and from there used multiple selective...

19 January 2021 8,365 4 View

What are the antimicrobial categories and agents that are currently considered to define a MDR, XDR and PDR Salmonella?

What antimicrobial categories and agents are currently considered to define non-typhoid Salmonella as multidrug resistant (MDR), extremely / extensively drug resistant (XDR) or pandrug resistant...

19 November 2020 755 3 View

What component/s of Listeria monocytogenes are strong enough to be the reason why it is hard to eliminate and resistant to antibiotics?

Listeria monocytogenes is a common foodborne pathogen. This bacteria is a strong pathogen and hard to eliminate and resistant to antibiotics even the newly created ones.

05 October 2020 6,953 3 View

How to knock out the gene of multidrug-resistant salmonella typhimurium?

During the knockout process, I found that my Salmonella was resistant to multiple antibiotics and could not screen successfully imported transgenes. Moreover, my bacteria seems to be very...

12 September 2020 6,683 1 View

How to compare growth rates for the same micro-organism in different media?

I have Growth rate data for Listeria monocytogenes the same range of temperatures in two different media. I want to know if the growth rates is significantly different between the media - 1) at...

10 September 2020 1,707 5 View

Determination of Lipid A in Salmonella?

Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....

26 August 2020 9,455 1 View

Are manufacturers obliged to identify all possible food contact surfaces for Listeria monocytogenes or an indicator organism testing?

Hi, Under 9 CFR 430, establishments in Alternative 2b and 3 are required to sample food contact surfaces for Listeria monocytogenes or an indicator organism....

10 March 2020 6,762 4 View