How to identify Peptide Concentration?

Any easy method to find custom peptide concentration? We have some synthesized peptides for our biotechnology and biochemistry based research, i am looking to find it's concentration. Anybody can...

04 January 2021 8,505 8 View

Can alcohol feeding treat respiratory infections in birds?

In discussion with some farmers and colleagues, I came to know that poultry birds are fed alcohols to enhance their production/performance and treat the respiratory infecions. Is there any...

13 December 2020 4,700 17 View

Can you suggest some South Asian journals ( Marketing focused) with good impact factor and indexing for a paper on Digital Marketing?

I have a paper titled "The impact of social media marketing on urban youths' brand loyalty". I am looking for potential South Asian journals Where I can publish this.

09 October 2020 9,308 12 View

Do I enter data of two groups in separate sheets in SPSS?

I am having trouble with using SPSS as I'm relatively new to this. I have two groups, control group and treatment group. Do I enter the data for each in separate sheets or do I create a variable...

01 October 2020 2,761 6 View

How antibodies are designed plz explain in step??

from where and how we select sequence of antibody for designing of antibody??i don't understand .kindly tell me.

19 September 2020 2,649 1 View

Anybody have JCR Impact Factor List 2020??

Greetings All I hope you are all fine. Please share the JCR impact factor list 2020 if anybody has. Thank You

18 June 2020 173 39 View

Importing CST to matlab ?

Hey guys! I was wondering if it's possible to import the radiation pattern of a microstrip circular patch from CST to Matlab. And Matlab try to compute the Array pattern with Kraus formula....

31 May 2020 6,615 3 View

Is it important to include ethics for a research proposal based on using MCF-7 cell line and Amylin in a laboratory?

Carrying a lab based test using MCF-7 cancer cell line and humanized Amylin requires approval from ethics? And if it does which type of ethics in specific?

23 January 2020 3,371 5 View

Deriving mathematical equation to improve the machine learning algorithm performance?

I am planning to do research in text classification but , how can I improve the performance of the chosen machine learning algorithm by enhancing classification through derivation of mathematical...

14 December 2019 441 3 View

Case study on classification?

Can anybody help me in finding simple case studies for classification of small data set, i,e applying all classifiers on a small dataset manually and explaining it.

10 December 2019 1,164 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View