I have a set of bacteria that I stained with fluorescent dyes to check for viability. The protocol suggests to place 5 ul of the suspension on a slide, cover with a cover glass, and observe on a fluorescent microscope.
The problem is that between the slide and the cover slip there is water and the cells are flowing around with the fluid.
Is there a way to immobilize the cells without changing the morphology of the cells or affecting the fluorescence?
I reckon that passing the slide on a Bunsen would do both. How about leaving the slides on air to dry? Or suspending the cells in glycerol or high pure agar?
Thank you
"Three microliters of concentrated cells were transferred onto an agarose pad containing 1.2% agarose and 20% LB medium for microscopy."
source: https://www.pnas.org/doi/10.1073/pnas.1311066110#sec-3
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