How to choose the stimulus for T-cell activation assays?

When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...

10 November 2019 3,080 3 View

What is the best way to interpret cell proliferation assay?

Hello I am optimising cell proliferation assay of human T cell using this dye from Thermofisher: https://www.thermofisher.com/order/catalog/product/65-0842-85 I have done two groups; one T cells...

02 March 2019 9,482 3 View

Is there a way to predict the binding epitope of an antibody?

Hello I have two antibodies for flow cytometry for the same antigen but they seem to bind differently as they give different percentages (one is low and the other is high). I don't know the...

01 February 2019 3,444 4 View

What are possible reasons for tumour cell line not killed by T-cells in vitro?

Hello, I work in cancer immunotherapy particularly in CAR T-cells. I have leukaemia cell lines such as KO-52, ksaumi and THP-1. They express my target antigen at different levels. My CARs are also...

05 June 2018 3,939 4 View

Why are the results variable between wells of same samples in protein assay?

04 May 2018 3,302 12 View

Should I do CD34 isolation of primary AML cells before flow staining?

Hello I have AML cells that have been cultured on stromal cells for weeks and would like to do flow staining looking for a specific antigen in the AML cells. I am not sure where these cells came...

31 December 2017 2,161 2 View

Rapid expansion protocol of T-cells principle?

08 September 2017 6,410 0 View

What are the cell lines that have intermediate and low levels of CD19 Expression?

Hello I am working on CD19-directed CAR T-cells and using CD19 expressing cell lines for cytokine and killing assays. I want to include different cell lines that have different levels of antigen...

05 June 2017 8,004 1 View

How difficult to transduce AML cell lines with luciferase?

02 March 2017 677 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

03 March 2021 5,360 2 View