10 Questions 5 Answers 0 Followers
Questions related from Abdulrahman Bahashwan
When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...
11 November 2019 3,126 3 View
Hello I am optimising cell proliferation assay of human T cell using this dye from Thermofisher: https://www.thermofisher.com/order/catalog/product/65-0842-85 I have done two groups; one T cells...
03 March 2019 9,545 3 View
Hello I have two antibodies for flow cytometry for the same antigen but they seem to bind differently as they give different percentages (one is low and the other is high). I don't know the...
02 February 2019 3,495 4 View
Hello I have a plasmid to transduce on T-cells. The sequence of this plasmid contains OM-1, which I know only it helps for protein expression, but how does it work I couldn't find an explanation...
09 September 2018 273 1 View
Hello, I work in cancer immunotherapy particularly in CAR T-cells. I have leukaemia cell lines such as KO-52, ksaumi and THP-1. They express my target antigen at different levels. My CARs are also...
06 June 2018 3,990 4 View
Hello there, I'm doing protein concentration for WB assay using BioRad DC™ Protein Assay similar principle to Lowry assay. I have 8 samples and standard.I do them in triplicates. However, I've...
05 May 2018 3,348 12 View
Hello I have AML cells that have been cultured on stromal cells for weeks and would like to do flow staining looking for a specific antigen in the AML cells. I am not sure where these cells came...
01 January 2018 2,203 2 View
Hello there, We work with genetically modified T-cells and use rapid expansion protocol to increase T-cell number. We use irradiated PBMCs from at least 3 different donors. I know some basic info...
09 September 2017 6,525 0 View
Hello I am working on CD19-directed CAR T-cells and using CD19 expressing cell lines for cytokine and killing assays. I want to include different cell lines that have different levels of antigen...
06 June 2017 8,060 1 View
Hello I'm trying to transduce some of AML cell lines with luciferase. The method I'm using is retrovirus transfection of 293T cells followed by transduction of the cells with help of Retronectin....
03 March 2017 740 3 View
