How to choose the stimulus for T-cell activation assays?

When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...

10 November 2019 3,080 3 View

What is the best way to interpret cell proliferation assay?

Hello I am optimising cell proliferation assay of human T cell using this dye from Thermofisher: https://www.thermofisher.com/order/catalog/product/65-0842-85 I have done two groups; one T cells...

02 March 2019 9,482 3 View

Is there a way to predict the binding epitope of an antibody?

Hello I have two antibodies for flow cytometry for the same antigen but they seem to bind differently as they give different percentages (one is low and the other is high). I don't know the...

01 February 2019 3,444 4 View

What is the function of OM-1 in gene expression?

Hello I have a plasmid to transduce on T-cells. The sequence of this plasmid contains OM-1, which I know only it helps for protein expression, but how does it work I couldn't find an explanation...

08 September 2018 207 1 View

What are possible reasons for tumour cell line not killed by T-cells in vitro?

Hello, I work in cancer immunotherapy particularly in CAR T-cells. I have leukaemia cell lines such as KO-52, ksaumi and THP-1. They express my target antigen at different levels. My CARs are also...

05 June 2018 3,939 4 View

Why are the results variable between wells of same samples in protein assay?

04 May 2018 3,302 12 View

Should I do CD34 isolation of primary AML cells before flow staining?

Hello I have AML cells that have been cultured on stromal cells for weeks and would like to do flow staining looking for a specific antigen in the AML cells. I am not sure where these cells came...

31 December 2017 2,161 2 View

What are the cell lines that have intermediate and low levels of CD19 Expression?

Hello I am working on CD19-directed CAR T-cells and using CD19 expressing cell lines for cytokine and killing assays. I want to include different cell lines that have different levels of antigen...

05 June 2017 8,004 1 View

How difficult to transduce AML cell lines with luciferase?

02 March 2017 677 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

03 March 2021 5,360 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View