What is the expected yield for plasma membranes isolated by sucrose gradient centrifugation from mouse tissues?

06 October 2015 1 5K Report

I'm wanting to isolate plasma membranes from mouse skeletal muscle to study GLUT4 translocation. Just curious about what an isolated muscle will give me. Downstream applications will be western blotting and radioligand binding.

Alfonsina Ramundo-Orlando

Dear Derek Bone,

we used rat liver about 40 g (from three adult rats) as starting material to obtain about 1.5 ml of plasma membrane suspension (we didn't weight the final pellet) containing an avarage of 10-15 mg/ml of protein (Lowry method).

If you need more details please don't hesitate to ask us.

Best regards Alfonsina Ramundo-Orlando

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