Can addition of glass modifier lower the solidus temperature of a glass composition?

In Li2O-SiO2 glass system a solidus line lies at 1033 deg C between lithium metasilicate + lithium disilicate (S) and lithium metasilicate + liquid (S+L). Can this solidus line be lowered to...

27 September 2020 5,742 3 View

How to measure refractive index of bulk glass sample from UV-VIS spectra?

I have recorded UV-VIS spectra in %R mode (in wavelength range of 200-900 nm) in bulk glass sample. How can I measure refractive index from this data? Thanks in advance, Santa

31 October 2019 4,351 4 View

How I could obtain ER positive normal breast cancer cell lines?

I can not find appropriate/satisfactory (scientific and research-based) information about ER+ normal breast cell line or normal cell line of other reproductive (or non-reproductive) tissue,...

06 July 2019 7,939 4 View

What are the basics to maintain a micropipette in good working conditions?

26 November 2014 9,393 4 View

Does anybody know the difference between GISTIC 2.0 and ABSOLUTE algorithms, if there is any?

While reading the literature,it is not very clear to me the rationale of using one or the another.

22 June 2014 4,609 1 View

What is the exact androgen receptor expression in MDA-MB-231 cells?

Could somebody help me with androgen receptor expression in MDA-MB-231 cells? I could not find the data about that.

09 January 2014 9,956 9 View

Could somebody sugest me an in vivo experiment for Inhibitory potential of compounds towards 17beta HSD 2 inhibition?

Some compounds inhibited this enzyme in in vitro tests. Do some 17beta HSD 2 inhibitors have medical/pharmaceutical application?

31 December 2013 9,980 3 View

How we can obtain or purchase Abiraterone or Abiraterone acetate?

We tried to purchase it in the pharmacy and the clinic. We needed it for research.

31 December 2013 2,460 2 View

What is the exact androgen receptor expression in MDA-MB-231 cells?

Could somebody help me with androgen receptor expression in MDA-MB-231 cells? I could not find the data about that.

31 December 2013 5,002 10 View

Dr.C Natalia, Please, Iwant to know more about the project?

I want to known, because I am microbiologist and Master in Virology, but I would like to work about Rabies Virus with you.

01 January 1970 6,228 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View