What is the estimated clearance time or half life of LPS in mice, when inhaled?

06 August 2021 1 2K Report

I have seen some literature discussing clearance of LPS from the bloodstream, where it can be cleared pretty well in about 6 hours (Article Lipopolysaccharide Is Cleared from the Circulation by Hepato...

)

What I want to get a better sense of, is how long would the inhaled LPS be in the lung for, before being cleared by cilia or the liver.

Ashten Stambersky

Hi Percival,

Here is some literature that may be helpful:

Article Elastase- and LPS-exposed mice display altered responses to ...

Article Chronic LPS Inhalation Causes Emphysema-Like Changes in Mous...

Hope this helps!

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Percival Graham's questions See All

Does anyone remember how to take a FISH image with Metasystems Isis software?

Does anyone remember how to take a FISH image with Metasystems Isis software and then take another at a different focal plane and combine them? I used to do this but now can't remember! Thanks

14 May 2024 10,025 0 View

What is the status of the principle of Justice in our legal system?

Oliver Wendel Holmes believed that justice had to do with customs that vary from one society to another, relativistic and without a foundation as an overarching principle. Marxist believe that...

22 March 2023 4,828 4 View

Contamination in primary bone marrow MSC culture?

Recently I’ve started seeing these clumps in my primary MSC flask (T175). I first noticed these compact clumps in a flask near 80% confluence but I’m also seeing it in less confluent flasks. The...

22 February 2023 9,528 3 View

Best means of quantifying cDNA for qPCR Input?

My lab has been using the Qubit ssDNA kit to quantify cDNA prior to qPCR input, but we have doubts about the accuracy of this kit. The MFR explicitly states that the Qubit ssDNA kit does not bind...

14 December 2022 1,052 2 View

Can we test one-directional hypotheses with ANOVA?

It is said that the p value for a t-test can be calculated as one-tailed or two-tailed. This makes sense as Condition A scores can be higher, or lower than Condition B scores (so a two-directional...

04 March 2022 3,629 4 View

What data is collected by Nurses in the Mobile Clinics in South Africa?

Traditionally patients in South Africa, particularly in rural areas retain their own clinical record, often in an exercise book or similar. Healthcare staff also record the history of a particular...

14 May 2021 3,930 3 View

Can artificial splice sites be used to create two transcripts?

Is it possible to have two protein variants transcribed from a transfected plasmid (or viral vector)? Not in the sense of using IRES or P2A/T2A, but rather in having two proteins with the same C...

02 March 2021 7,761 2 View

Can anyone suggest various protocols/stains for culturing and imaging Myxamoebae using a light microscope?

All suggestions welcome. We are curious to find out what works well, and to hear about diverse methodology. Thanks! We currently have restricted access to a lab where Silver staining etc could...

09 June 2020 9,263 3 View

UK Practice MRI Knee : what factors from the radiology report are used in decision making for arthroscopy ?

If an MRI knee scan identifies a meniscal tear; what factors are taken into consideration to send for arthroscopy? If the scan has been generated from a GP referral: is there vetting to decide...

14 May 2020 352 1 View

Where can I find studies on psychopathology and left-handedness, specifically schizophrenia and PTSD?

I am attempting to write a paper on the relationship between left-handedness and psychopathology, including PTSD, bipolar disorder and schizophrenia.

13 March 2020 9,783 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View