All suggestions welcome. We are curious to find out what works well, and to hear about diverse methodology. Thanks!
We currently have restricted access to a lab where Silver staining etc could be conducted.
What we have tried:
H&E
KOH
Lactophenol Cotton Blue
Alcian Blue
Calcofluor White
Safranin O
Chigago Sky Blue
Biskmark Brown
Crystal Violet
India Ink
Malachite Green
Aggar Culture
Myxamoebae, social amoebae, slime mold, plasmodium, fungal culture, mycology, microscopy protocol, oomycetes, oomycota
For culturing you can go for 1.5 water agar. I have been cultivating myxomycetes in laboratory and found 1.5 water agar giving best results.
You can try
1. Carnoy's solution for fixation of myxamoebae, then stain with 2% Gurr's acetoorcein in 45% acetic acid following hydration through alcohol series and hydrolysis in 1N HCl (Sussman R, 1961).
2. Fix the cells in methanol/glacial acetic acid (3:1, v/v), then stain with 10% Gurr's giemsa stain in Sorenson's phosphate buffer (ph-6.8) (Brody and Williams, 1974).
These methods will be helpfull when you want to stain nuclei of vegetative myxamoebae. Hope it helps.
@ Preeti Vinayak Phate and @ Abhishek Dharm Singh , Thank you for your suggestions, we will try them out.
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