Hi everyone,
what is the voltage of the electrical potential of the cell membrane when a seizure starts in epileptic patients?
and what is it in the normal cell? ( before seizure)
I want to know the exact difference between these two?
what is the protocol for enzyme freeze drying?
09 July 2024 6,464 6 View
In Silvaco, I have defined a semiconductor component. The electric field outputs have two problems. One is that the steps of X are not the same and they change, although I have defined a...
19 March 2024 4,649 0 View
The CASTEP in my case runs without materials studio. I want to calculate the band structure and optical properties of a perovskite material in this CASTEP code. I want to know how to write the...
28 February 2024 5,694 2 View
The Tianjin-Yonghe's cable Bridge in china The repair of this bridge was done between 2005 and 2007
07 January 2024 3,940 0 View
Improvement through visualization
22 November 2023 5,807 0 View
I'm facing challenges with the purity of plasmid DNA extracted from Trypanosoma brucei for PCR applications. Despite using a commercial DNA extraction kit, I suspect the presence of contaminants...
20 October 2023 829 3 View
Any structures like the bridge, building,..... It is better if the structure is reinforced concrete. For damage detection using machine learning.
15 September 2023 4,129 3 View
Hi every body I made the ship's environment based on M9 culture media with the following ingredients( per 100 ml culture media ): KCL= 0.2 gr NaCl= 0.01 gr KH2PO4 =0.2 gr MgSO4= 0.001 gr Fe so4...
16 May 2023 8,115 0 View
Dear Sir or Madam I simulated a 3D PIN photodiode in SILVACO ATLAS. It stops in "material" and has an error: " Memory allocation failure. Try killing processes or see system administrator". There...
29 March 2023 7,621 3 View
I have done DFT calculation of a perovskite material (KGeI3) with CASTEP code. I want to find out its electron affinity, effective conduction or valance band density of states and relative...
17 March 2023 5,912 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View