Hi every body
I made the ship's environment based on M9 culture media with the following ingredients( per 100 ml culture media ):
KCL= 0.2 gr
NaCl= 0.01 gr
KH2PO4 =0.2 gr
MgSO4= 0.001 gr
Fe so4 =0.001 gr
Znso4 =0.001 gr
fenol red=( 0.001 gr per 100 ml)
L-arginine= 1 gr
Why, despite the correct percentages of each of the materials, does the culture medium precipitate regularly? What is the solution to this problem?
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