How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

What online teaching new topics to address as a phd topic?

Greetings, I have just started my phd in the field of english language studies, Im currently interested in online teaching, e-learning but mostly english language pedagogy. what are the new...

16 November 2020 4,045 9 View

Hi There any one can help on the case study below?

04 October 2020 7,234 1 View

After COVID 19, Can health be a decisive point for urban regeneration?

Will there be a return to suburbia after social distancing in city centres? From NY to Bombay how will people react to density and habitations after the tiny assassins take over us?

05 May 2020 6,256 6 View

Best strategy to transfect large genes in mammalian cells?

I have an insert ( ORF 6,9kb long) that I want to transfect in a human colon cancer cell line. I initially wanted to do with lentivirus, since I could have a stable expression of my gene and...

22 April 2020 3,277 3 View

What to do with coincident features for Geographically weighted regression?

I am sorry if this is a simple question. I am trying to conduct a GWR for hedonic analysis of hotel room rates. For OLS I have conducted analysis including different room types (e.g. double,...

16 April 2020 7,062 3 View

Can a conjugated isotype antibody be used in cell culture function blocking antibody experiment?

I have a PE conjugated mouse k isotype antibody..I would like to use it as control and add to cell culture .I would like to know whether conjugate will interfere in cell culture...

22 January 2020 607 2 View

What should be a loading control in condition media for zymography?

I want to do a zymography in condition media from cancer cell line...I would like to know what should be a loading control for condition media specific for zymography? Whether loading control can...

13 November 2019 1,697 1 View

Why c18 column isn't working?

I tried to work using same method I have worked before. But now not getting any peak. I am confused. Unable to understand what's happening. What can I do now?

02 October 2019 9,790 6 View

What is the best way to analyze interview question with scale answer from 1-5?? could I use likert even my research is qualitative?

I have a structured interview to test a hypothesis, one of the questions required an answer from 1 to 5 .. I don't know how to analyze this? any recommendations? @qulitative analysis @Structured...

21 February 2019 6,307 11 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View