Excluding IHC
We can use qPCR, FISH, immunocytochem, flow cytometry and maybe some more simple methods
dear Mariia,
the choice depends also on the throughput you need... have you got 10 patients, 100 or more?
You may decide to screen for mutations one exon at a time with methods such as DGGE or SSCP, or to PCR amplify exons (including intron-exon junctions) and sequence all of them, or focus on the exons encoding the DBD. Of course this means that most axons will turn out to be wild type. Having access to NGS may accelerate the process. But you have to think about the likelihood of finding a mutation and also about the sensitivity of each technique in identifying a mutation that may be in a minority of cells in the tumor.
Good luck!
Paola
We have all this samples without any TP53 mutation. And we need to study functional disorders. And our technical capabilities are limited
Perhaps Western-blotting with AB for phospho-p53 could be used.
- Badges
- Science method