What is the drug standard used in research for EGRF mutated lung cancer cell line?

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How to determine the Interaction Volume in Elastic Nanoindentation?

I am interested in methods/equations for calculating the interaction volume during elastic nanoindentation. Specifically, I'm interested in determining how much of the material (other than the...

29 January 2024 5,318 2 View

What are thermal properties of Poly (p-toluene sulphonate) polydiacetylene PTS (PDA)?

Hello everyone, Could anyone tell me the values of themal conductivity, mas density and heat capacity of Poly (p-toluene sulphonate) polydiacetylene PTS? I need these parameters in my heat...

03 September 2023 2,297 0 View

Why the baseline of DSC measurements is not flat?

I am performing some DSC (perkin elmer 8500) measurements (same materials, same mass, same program). Following calibration, I encountered different baselines every day. The baseline was flat only...

02 July 2023 2,443 9 View

Does DSC horizontal mode in DSC 404 F3 NETZSCH affect my analysis??

My DSC curve (Figure1) do not show a horizontal shape. It may be related to the calibration, even though we recently calibrated the instrument. In any case, the software offers an option in the...

16 June 2022 258 4 View

Do you have any suggestions for MATLAB code for fractional wavelet transform?

Although the FRFT has a number of unique properties, it cannot obtain information about local properties of the signal. In addition, the drawback of the short-time FRFT is that its time- and...

26 June 2021 7,322 2 View

What are the advantages and disadvantages of fractional S transform versus fractional Fourier transform?

Fractional transforms not only enhance the overall signal processing applications but also improved the efficacy of systems.

04 January 2021 8,629 4 View

Please a python code for estimation of Lyapunov exponent?

One of the most striking features for deterministic chaotic systems is the limited predictability (or unpredictability) of the future evolution of the system, despite the determinism of the...

12 August 2020 6,951 3 View

Which algorithm (Sobel, Perwitt, Roberts, Canny and LOG algorithm) is more capable of finding noisy image edges?

The edge detection algorithms are designed to respond to sharp edges which can be caused by noisy pixels. In image analysis edge detection plays an important role and it is one of the traditional...

05 August 2020 2,758 1 View

Which normalization algorithm (min–max, z-score, andtanh normalization) has the highest score in correct time series similarity?

Although information fusion in a multimodal system can be performed at various levels, integration at the matching score level is the most common approach due to the ease in accessing andcombining...

05 August 2020 2,704 0 View

What algorithm is better than the DTW algorithm to show the similarity of time series?

Dynamic Time Warping (DTW) is one of the important distance measures for time series, however, the exact calculation of DTW has become a bottleneck.

04 August 2020 4,975 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View