which one is better in performing Loop mediated isothermal amplification (LAMP)
Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?
07 August 2018 8,943 3 View
I need to analyze my data statistically, and I need a user friendly software with international recognition for publication, please suggest any software for that purpose. It would be great help if...
06 July 2018 3,888 12 View
If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc
11 December 2017 10,081 1 View
I have seen following...
11 December 2017 1,280 4 View
should we publish in Journals having GIF, as they are charging very few amount of fee.
10 November 2017 7,949 0 View
Is this journal recognized or not? it is showing that it is indexed in Index Copernicus.
10 November 2017 4,813 6 View
What is best method for enhancing the expression of a gene if you need a protein, is it increasing copy no or removing control from it or anything else?
10 November 2017 7,366 5 View
I used to use PCR primers even after 3-4 months and they worked, but now something seems to be wrong with them, they stop working after few runs. I am totally clueless about it.
09 October 2017 1,507 6 View
I need to work on expression of a gene. can anyone tell me about the difference of these primers?
09 October 2017 6,608 4 View
when we use reverse transcriptase do we have to use specific buffer with that or any taq buffer can also work with that?
09 October 2017 1,745 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...
03 August 2024 6,500 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View