What is the difference in Bst DNA Polyemrase and Bsm DNA Polymerase?

01 January 1970 0 7K Report

which one is better in performing Loop mediated isothermal amplification (LAMP)

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What is the proper method for cleaning of combs for agarose gel electrophoresis?

Can we wash it by boiling it in water if the visible stains of ethidium bromide are not washed after rinsing with water and then 70% ethanol?

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Which software is better for Statistical Analysis, R or SPSS?

I need to analyze my data statistically, and I need a user friendly software with international recognition for publication, please suggest any software for that purpose. It would be great help if...

06 July 2018 3,787 12 View

What does number written between sequence of nucleotide means?

If there is a number superscript on the sequence of a primer what does it means? e.g. T153AGCT etc

11 December 2017 9,986 1 View

Can anyone recommend me any tools or software's for Viral In silico PCR and predicting DNA bands?

I have seen following...

11 December 2017 1,164 4 View

Does anyone know what is Global Impact Factor (GIF)?

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10 November 2017 7,851 0 View

Does anyone know about Academic Research International Journal?

Is this journal recognized or not? it is showing that it is indexed in Index Copernicus.

10 November 2017 4,699 6 View

What is the best method for Enhancing Gene Expression?

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10 November 2017 7,263 5 View

What is the difference between normal primers and primers for cloning?

I need to work on expression of a gene. can anyone tell me about the difference of these primers?

09 October 2017 6,390 4 View

How quickly the PCR primer deteriorates?

I used to use PCR primers even after 3-4 months and they worked, but now something seems to be wrong with them, they stop working after few runs. I am totally clueless about it.

09 October 2017 1,404 6 View

My PCR band disappeared after optimization, what should I do?

I ran a PCR reaction and got my desired band at 800 bp with other non specific bands (reaction vol 25 ul) after optimization I got 800 bp band clear (reaction vol 25 ul). Then I went for bulk...

09 October 2017 6,435 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

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26 February 2021 8,399 5 View