25 August 2023 0 2K Report

Hello!

I am new to the field of research involving blood samples, and in particular, I am interested in working with menstrual blood. My goal is to understand the protocol for processing blood samples using Ficoll to isolate different cell populations. I would greatly appreciate it if someone with experience could guide me on some key aspects of the process.

Questions:

  • Pre-washing before cell isolation with Ficoll: We receive our blood samples mixed with a medium containing antibacterial and antifungal agents. In this case, would it be necessary to perform any pre-washing steps on the blood samples before proceeding to isolate cells using the Ficoll protocol? If so, what pre-washing approach is recommended and with which solution?
  • Centrifugation in the Ficoll protocol: In the Ficoll protocol, could you provide information on the recommended centrifugation speed (in g) and the appropriate duration to separate different cell populations? Are there recommended variations depending on the type of blood sample, such as menstrual blood?
  • Stability and storage of cell populations: We are receiving our blood samples 48-72 hours after the collection. I am particularly interested in understanding the viability of the cells, especially in the case of menstrual blood (we are especially interested in Menstrual Stem Cells). Is it known whether the different cell populations can maintain their integrity and function when stored at temperatures around 4°C for a period of 48-72 hours? This information would be valuable for planning my experiments and sample processing.

    • I sincerely appreciate any guidance and advice you can provide!

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