Well, the Taqmanprobe contains a fluorophore and a quencher. This quencher is degraded when this primer did bind to your DNA. What is left is the fluorophore which gets measured in the system. With SYBRgreen it is a completely different story: It is a normal primer without any tags and the fluorophore in your SYBRgreen solution intercalates beetween your amplified DNA double strands. As simply as this. You could use the SYBRgreen primers as Taqman probes but you need to tag them with a fluorophore on one side and a quencher on the other. I guess that might be too difficult.

Hi Shyam,

In Additional to @Teresa Otto, the Taqman probe based PCR is more accurate and less prone to the errors caused by the contaminants which can cause the fluorescence interference. The probe based method also gives the possibility of multiplexing which is not possible in the intercalation based PCR assays. Thus, this method is more dynamic and accurate but also expensive. Developing the probes is hard and expensive as compared to the normal primers used in the SYBR based assays.

Hello Shyam,

There're few imp. criteria to consider for Taqman probes. These could be informative in detail.

PREMIER Biosoft: http://www.premierbiosoft.com/tech_notes/TaqMan.html

Thermofisher manual:

https://tools.thermofisher.com/content/sfs/manuals/cms_042307.pdf

Good Luck!

There are some key differences between two in terms of Gene expression, Reproducibility, Specificity, application purposes etc. However, the following links could be informative.

@Surya Prasad Tiwari

Hi Sir,

Thanks for your valuable information. I will look into it.

Shyam

@Abhijeet Singh

@Teresa Otto

Hi,

Thank you very much for your kind support and informative answers.

Shyam

Dye-based assays are perfect for many real-time qPCR applications. When you understand the true advantages and disadvantages of probe-based assays, you can reduce the cost while keeping the quality of your qPCR experiments high.

Novice qPCR users often argue that data generated with dye-based assays are susceptible to misinterpretation: the detection of nonspecific PCR products would influence the Cq values.

But the detection of nonspecific PCR products should not be cancelled out by adding a probe to the qPCR reaction. It is through careful experiment design and optimization that you obtain specific, efficient and sensitive amplification and detection of your target of interest. Primer concentrations, denaturation and annealing times and temperatures, and buffer composition are few of many critical parameters that contribute to a robust qPCR reaction. When sensitivity is not an aspect that you want to improve, optimizing these parameters will give you a suitable qPCR assay. And it does not need a probe. This is the strategy applied by Biogazelle when designing Bio-Rad's PrimePCR assays: the most comprehensive portfolio of validated Research Use Only dye-based qPCR assays for human, mouse and rat.

You can find additional arguments, and additional advice on when to use what detection technology at https://blog.qbaseplus.com/taqman-probe-or-sybr-green-dye